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The use of fiber optics also aids in separating the high-intensity light from its source which can generate radiant heat that would otherwise quickly raise the temperature within the experimental filming vessel.The camera exposure was set to 10 µs which provided suitable image brightness and was also sufficient to eliminate particle streaking that can be caused by a continuous illumination source and fast animal motion.This can improve our understanding of animal–fluid interactions at small spatial scales and low Reynolds number.Particle image velocimetry (PIV) has become an important and widely used tool in biological research involving fluid motion.For lower magnification applications (≤×10), were grown in a temperature controlled, illuminated incubator (Thermo Scientific) set to a 12 : 12 light cycle and grown in f/2 media at 20°C.Appropriate densities of algae were obtained through 5 min of centrifuging followed by re-suspension in filtered seawater.

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The velocities of particles within the focal plane were determined from sequential images analyzed using a cross-correlation algorithm (La Vision software).

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